Solution to Activity 6.3 of NCERT Class 9 Science Chapter 6 Tissues

3. Complete Activity 6.3 (Page 63).

• Take a freshly plucked leaf of Rhoeo.

• Stretch and break it by applying pressure.

• While breaking it, keep it stretched gently so that some peel or skin projects out from the cut.

• Remove this peel and put it in a petri dish filled with water.

• Add a few drops of safranin.

• Wait for a couple of minutes and then transfer it onto a slide. Gently place a cover slip over it.

• Observe under microscope.

Answer:

Aim: To observe the peel of Rhoeo leaf under microscope and conclude based on that.

Materials Required: Rhoeo leaf, petri dish, water, safranin, slide, cover slip, microscope.

Procedure:

(i) The Rhoeo leaf is stretched and broken by applying pressure. While breaking the leaf, it is stretched gently so that some peel or skin projects out from the cut.

(ii) The peel is removed and put in a petri dish filled with water.

(iii) A few drops of safranin are added to it.

(iv) After a couple of minutes it is transferred to a slide, a cover slip is placed over it and it is observed under a microscope.

Observations:

The cells are flat and form a continuous layer without intercellular spaces.
Small pores are present here and there.

Epidermis of Rhoeo leaf viewed under microscope. — **Epidermis:** The outermost layer of cells called epidermis of Rhoeo leaf is shown above.

Conclusions:

We observed the outermost layer of cells known as the epidermis.
The epidermis is made of a single layer of flat cells (called epidermal cells), form a continuous layer without intercellular spaces since it offers protection.
The small pores observed are called stomata, which are enclosed by two kidney-shaped cells called guard cells.

“Take a freshly plucked leaf of Rhoeo.

Stretch and break it by applying pressure.

While breaking it, keep it stretched gently so that some peel or skin projects out from the cut.

Remove this peel and put it in a petri dish filled with water.

Add a few drops of safranin.

Wait for a couple of minutes and then transfer it onto a slide. Gently place a cover slip over it.

Observe under microscope.” – Solved.